AMERICAN ANGUS ASSOCIATION - THE BUSINESS BREED

Submitting Samples

Tissue Sampling Unit (TSU) -

Preferred Method

(Acceptable for twins)

1.) Ensure the tissue applicator is loaded with an unused tissue punch (red plunger should be visible). Order Online.

2.) Slide gun over the ear, and position the cutter approximately 1 inch from the edge of the ear, taking care to avoid any obvious veins and ridges.

3.) Squeeze handles together to take a sample and then release to free the ear. Try to do this in one swift, fluid, motion.

4.) Remove the punch from the device and check that sampling has been successful. If not, discard the sample and re-sample with a new punch.

5.) Remove the used cutter from the applicator by pulling the handles apart. This will loosen the cutter. Discard safely as cutter is very sharp.

6.) Record animal id (tattoo, tag, etc.) and reference barcode.

Submission Instructions for TSUs

Once the tissue sample has been successfully extracted, care should be taken to store the sample at room temperature and away from sunlight for up to a year. We recommend freezing the sample after a year, for long term storage.

Blood Sample -

Preferred Method

1.) Record animal ID # (tattoo and/or tag number) on a DNA card. Order Online.

2.) Wipe the ear clean using a clean paper towel or cloth.

3.) Prick the vein in the animal’s ear with a sterile needle.

4.) Touch the circle on the DNA card to the blood site on the ear.

5.) Fill the circle with blood.

6.) Allow the card to dry away from direct sunlight for at least 24 hours prior to mailing. Do not mail the card in a sealed plastic bag.

Good Sample

Bad Samples

Contaminated with feces
Not enough blood

Hair Sample -

(Acceptable for twins. Not recommended for animals younger than 4 months.)

1.) Record animal ID #(tattoo and/or tag number) on the hair card.

2.) Pull from the tail switch “up and away” (20 hairs with root bulbs).

3.) Place bulbs on the back flap. Peel off backing paper on sticky flap.

4.) Press sticky side on top of bulbs to seal sides. Hair bulbs must be present to perform tests.

5.) Trim excess hair to the edges of the hair card.

Good Sample

Bad Samples
Excess hair not trimmed
No root ball
Additional Sampling Techniques
Semen Sample
1.)Insert thawed semen straw into a writing pen with the ink tube removed.
2.)Indicate animal ID #(tattoo and/or tag number) on the straw of semen.
3.)Send at room temperature.
4.)Ship in a padded envelope or small box.

Blood Tube Sample
1.)Mark purple-top tube with the animal ID #(tattoo and/or tag number). Fill the tube with 6-9 mL of blood.
2.)Refrigerate promptly in a stand-up rack between 4-10° C (39.2 - 50° F). Do not freeze.
3.)Ship samples overnight with a cold pack in an insulated cooler or container.
Tissue Sample (Deceased animals only) Call for instructions

Printable Version

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